Machine Learning

Structural heterogeneity in single-particle images presents a major challenge for high-resolution cryo-electron microscopy (cryo-EM) structure determination. We recently developed a statistical manifold learning approach for unsupervised single-particle deep classification. When optimized for Intel high-performance computing ...... Read more

Watching protein degradation

The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is responsible for selective protein degradation in eukaryotic cells. We determined cryo-electron microscopy structures of the substrate-engaged human proteasome in seven conformational states at 2.8-3.6 angstrom resolution, captured during ...... Read more